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ATCC human mesenchymal stromal cells
a) In vitro adipogenesis model. <t>Mesenchymal</t> stromal cells (MSCs) were plated and cultured in either regular or differentiation medium for indicated durations. b) Representative image of PPARG RNA FISH in undifferentiated MSCs (Day 0) and adipocytes (Day 14). c) Proportion of nuclei exhibiting bursts of transcription on one (monoallelic) or two of the alleles (biallelic) across four timepoints. d) Left: number of transcript-intensity equivalents per burst at each active transcription site. Right: total number of nuclear transcripts per nucleus. * P < 0.05, ** P <0.01. *** P <0.001. e) Top: H3K27ac ChIP-seq tracks of the PPARG locus during adipogenic differentiation (range: 0-30 for all tracks); bottom: Genomic target regions labeled by DNA FISH in four colors. f) Representative maximum intensity projection of DNA FISH data (downstream control locus not shown). g) Pairwise 3D distances between genomic regions of interest measured by DNA FISH and corrected using BeadBuddy. Empirical cumulative distribution functions visualizing changes in distance between specified genomic elements as a function of differentiation timepoint (average of two technical replicates for each timepoint). h) Spatial density heatmaps: each datapoint is an absolute distance of the promoter from the midpoint of the two enhancers on that promoter’s allele. Green and purple ovals represent a distribution of enhancer positions relative to the midpoint (the origin) where each oval’s long axis is 1 standard deviation of enhancer distance from the midpoint. Detailed Statistics are presented in Supplementary Table 1.
Human Mesenchymal Stromal Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
CLS Cell Lines Service GmbH human bone marrow stromal cells mscs
a) In vitro adipogenesis model. <t>Mesenchymal</t> stromal cells (MSCs) were plated and cultured in either regular or differentiation medium for indicated durations. b) Representative image of PPARG RNA FISH in undifferentiated MSCs (Day 0) and adipocytes (Day 14). c) Proportion of nuclei exhibiting bursts of transcription on one (monoallelic) or two of the alleles (biallelic) across four timepoints. d) Left: number of transcript-intensity equivalents per burst at each active transcription site. Right: total number of nuclear transcripts per nucleus. * P < 0.05, ** P <0.01. *** P <0.001. e) Top: H3K27ac ChIP-seq tracks of the PPARG locus during adipogenic differentiation (range: 0-30 for all tracks); bottom: Genomic target regions labeled by DNA FISH in four colors. f) Representative maximum intensity projection of DNA FISH data (downstream control locus not shown). g) Pairwise 3D distances between genomic regions of interest measured by DNA FISH and corrected using BeadBuddy. Empirical cumulative distribution functions visualizing changes in distance between specified genomic elements as a function of differentiation timepoint (average of two technical replicates for each timepoint). h) Spatial density heatmaps: each datapoint is an absolute distance of the promoter from the midpoint of the two enhancers on that promoter’s allele. Green and purple ovals represent a distribution of enhancer positions relative to the midpoint (the origin) where each oval’s long axis is 1 standard deviation of enhancer distance from the midpoint. Detailed Statistics are presented in Supplementary Table 1.
Human Bone Marrow Stromal Cells Mscs, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
human bone marrow stromal cells mscs - by Bioz Stars, 2026-02
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96
ATCC human bone marrow mesenchymal stem
a) In vitro adipogenesis model. <t>Mesenchymal</t> stromal cells (MSCs) were plated and cultured in either regular or differentiation medium for indicated durations. b) Representative image of PPARG RNA FISH in undifferentiated MSCs (Day 0) and adipocytes (Day 14). c) Proportion of nuclei exhibiting bursts of transcription on one (monoallelic) or two of the alleles (biallelic) across four timepoints. d) Left: number of transcript-intensity equivalents per burst at each active transcription site. Right: total number of nuclear transcripts per nucleus. * P < 0.05, ** P <0.01. *** P <0.001. e) Top: H3K27ac ChIP-seq tracks of the PPARG locus during adipogenic differentiation (range: 0-30 for all tracks); bottom: Genomic target regions labeled by DNA FISH in four colors. f) Representative maximum intensity projection of DNA FISH data (downstream control locus not shown). g) Pairwise 3D distances between genomic regions of interest measured by DNA FISH and corrected using BeadBuddy. Empirical cumulative distribution functions visualizing changes in distance between specified genomic elements as a function of differentiation timepoint (average of two technical replicates for each timepoint). h) Spatial density heatmaps: each datapoint is an absolute distance of the promoter from the midpoint of the two enhancers on that promoter’s allele. Green and purple ovals represent a distribution of enhancer positions relative to the midpoint (the origin) where each oval’s long axis is 1 standard deviation of enhancer distance from the midpoint. Detailed Statistics are presented in Supplementary Table 1.
Human Bone Marrow Mesenchymal Stem, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human bone marrow mesenchymal stem/product/ATCC
Average 96 stars, based on 1 article reviews
human bone marrow mesenchymal stem - by Bioz Stars, 2026-02
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96
ATCC mesenchymal stromal cells mscs
a) In vitro adipogenesis model. <t>Mesenchymal</t> stromal cells (MSCs) were plated and cultured in either regular or differentiation medium for indicated durations. b) Representative image of PPARG RNA FISH in undifferentiated MSCs (Day 0) and adipocytes (Day 14). c) Proportion of nuclei exhibiting bursts of transcription on one (monoallelic) or two of the alleles (biallelic) across four timepoints. d) Left: number of transcript-intensity equivalents per burst at each active transcription site. Right: total number of nuclear transcripts per nucleus. * P < 0.05, ** P <0.01. *** P <0.001. e) Top: H3K27ac ChIP-seq tracks of the PPARG locus during adipogenic differentiation (range: 0-30 for all tracks); bottom: Genomic target regions labeled by DNA FISH in four colors. f) Representative maximum intensity projection of DNA FISH data (downstream control locus not shown). g) Pairwise 3D distances between genomic regions of interest measured by DNA FISH and corrected using BeadBuddy. Empirical cumulative distribution functions visualizing changes in distance between specified genomic elements as a function of differentiation timepoint (average of two technical replicates for each timepoint). h) Spatial density heatmaps: each datapoint is an absolute distance of the promoter from the midpoint of the two enhancers on that promoter’s allele. Green and purple ovals represent a distribution of enhancer positions relative to the midpoint (the origin) where each oval’s long axis is 1 standard deviation of enhancer distance from the midpoint. Detailed Statistics are presented in Supplementary Table 1.
Mesenchymal Stromal Cells Mscs, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mesenchymal stromal cells mscs/product/ATCC
Average 96 stars, based on 1 article reviews
mesenchymal stromal cells mscs - by Bioz Stars, 2026-02
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96
ATCC umbilical derived mesenchymal stem
a) In vitro adipogenesis model. <t>Mesenchymal</t> stromal cells (MSCs) were plated and cultured in either regular or differentiation medium for indicated durations. b) Representative image of PPARG RNA FISH in undifferentiated MSCs (Day 0) and adipocytes (Day 14). c) Proportion of nuclei exhibiting bursts of transcription on one (monoallelic) or two of the alleles (biallelic) across four timepoints. d) Left: number of transcript-intensity equivalents per burst at each active transcription site. Right: total number of nuclear transcripts per nucleus. * P < 0.05, ** P <0.01. *** P <0.001. e) Top: H3K27ac ChIP-seq tracks of the PPARG locus during adipogenic differentiation (range: 0-30 for all tracks); bottom: Genomic target regions labeled by DNA FISH in four colors. f) Representative maximum intensity projection of DNA FISH data (downstream control locus not shown). g) Pairwise 3D distances between genomic regions of interest measured by DNA FISH and corrected using BeadBuddy. Empirical cumulative distribution functions visualizing changes in distance between specified genomic elements as a function of differentiation timepoint (average of two technical replicates for each timepoint). h) Spatial density heatmaps: each datapoint is an absolute distance of the promoter from the midpoint of the two enhancers on that promoter’s allele. Green and purple ovals represent a distribution of enhancer positions relative to the midpoint (the origin) where each oval’s long axis is 1 standard deviation of enhancer distance from the midpoint. Detailed Statistics are presented in Supplementary Table 1.
Umbilical Derived Mesenchymal Stem, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
ATCC mesenchymal stromal cells
a) In vitro adipogenesis model. <t>Mesenchymal</t> stromal cells (MSCs) were plated and cultured in either regular or differentiation medium for indicated durations. b) Representative image of PPARG RNA FISH in undifferentiated MSCs (Day 0) and adipocytes (Day 14). c) Proportion of nuclei exhibiting bursts of transcription on one (monoallelic) or two of the alleles (biallelic) across four timepoints. d) Left: number of transcript-intensity equivalents per burst at each active transcription site. Right: total number of nuclear transcripts per nucleus. * P < 0.05, ** P <0.01. *** P <0.001. e) Top: H3K27ac ChIP-seq tracks of the PPARG locus during adipogenic differentiation (range: 0-30 for all tracks); bottom: Genomic target regions labeled by DNA FISH in four colors. f) Representative maximum intensity projection of DNA FISH data (downstream control locus not shown). g) Pairwise 3D distances between genomic regions of interest measured by DNA FISH and corrected using BeadBuddy. Empirical cumulative distribution functions visualizing changes in distance between specified genomic elements as a function of differentiation timepoint (average of two technical replicates for each timepoint). h) Spatial density heatmaps: each datapoint is an absolute distance of the promoter from the midpoint of the two enhancers on that promoter’s allele. Green and purple ovals represent a distribution of enhancer positions relative to the midpoint (the origin) where each oval’s long axis is 1 standard deviation of enhancer distance from the midpoint. Detailed Statistics are presented in Supplementary Table 1.
Mesenchymal Stromal Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mesenchymal stromal cells/product/ATCC
Average 96 stars, based on 1 article reviews
mesenchymal stromal cells - by Bioz Stars, 2026-02
96/100 stars
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90
Lonza human: primary mesenchymal stromal cells
a) In vitro adipogenesis model. <t>Mesenchymal</t> stromal cells (MSCs) were plated and cultured in either regular or differentiation medium for indicated durations. b) Representative image of PPARG RNA FISH in undifferentiated MSCs (Day 0) and adipocytes (Day 14). c) Proportion of nuclei exhibiting bursts of transcription on one (monoallelic) or two of the alleles (biallelic) across four timepoints. d) Left: number of transcript-intensity equivalents per burst at each active transcription site. Right: total number of nuclear transcripts per nucleus. * P < 0.05, ** P <0.01. *** P <0.001. e) Top: H3K27ac ChIP-seq tracks of the PPARG locus during adipogenic differentiation (range: 0-30 for all tracks); bottom: Genomic target regions labeled by DNA FISH in four colors. f) Representative maximum intensity projection of DNA FISH data (downstream control locus not shown). g) Pairwise 3D distances between genomic regions of interest measured by DNA FISH and corrected using BeadBuddy. Empirical cumulative distribution functions visualizing changes in distance between specified genomic elements as a function of differentiation timepoint (average of two technical replicates for each timepoint). h) Spatial density heatmaps: each datapoint is an absolute distance of the promoter from the midpoint of the two enhancers on that promoter’s allele. Green and purple ovals represent a distribution of enhancer positions relative to the midpoint (the origin) where each oval’s long axis is 1 standard deviation of enhancer distance from the midpoint. Detailed Statistics are presented in Supplementary Table 1.
Human: Primary Mesenchymal Stromal Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human: primary mesenchymal stromal cells/product/Lonza
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a) In vitro adipogenesis model. Mesenchymal stromal cells (MSCs) were plated and cultured in either regular or differentiation medium for indicated durations. b) Representative image of PPARG RNA FISH in undifferentiated MSCs (Day 0) and adipocytes (Day 14). c) Proportion of nuclei exhibiting bursts of transcription on one (monoallelic) or two of the alleles (biallelic) across four timepoints. d) Left: number of transcript-intensity equivalents per burst at each active transcription site. Right: total number of nuclear transcripts per nucleus. * P < 0.05, ** P <0.01. *** P <0.001. e) Top: H3K27ac ChIP-seq tracks of the PPARG locus during adipogenic differentiation (range: 0-30 for all tracks); bottom: Genomic target regions labeled by DNA FISH in four colors. f) Representative maximum intensity projection of DNA FISH data (downstream control locus not shown). g) Pairwise 3D distances between genomic regions of interest measured by DNA FISH and corrected using BeadBuddy. Empirical cumulative distribution functions visualizing changes in distance between specified genomic elements as a function of differentiation timepoint (average of two technical replicates for each timepoint). h) Spatial density heatmaps: each datapoint is an absolute distance of the promoter from the midpoint of the two enhancers on that promoter’s allele. Green and purple ovals represent a distribution of enhancer positions relative to the midpoint (the origin) where each oval’s long axis is 1 standard deviation of enhancer distance from the midpoint. Detailed Statistics are presented in Supplementary Table 1.

Journal: bioRxiv

Article Title: BeadBuddy: user-friendly, nanometer-scale registration of single-molecule imaging data

doi: 10.1101/2025.11.25.690467

Figure Lengend Snippet: a) In vitro adipogenesis model. Mesenchymal stromal cells (MSCs) were plated and cultured in either regular or differentiation medium for indicated durations. b) Representative image of PPARG RNA FISH in undifferentiated MSCs (Day 0) and adipocytes (Day 14). c) Proportion of nuclei exhibiting bursts of transcription on one (monoallelic) or two of the alleles (biallelic) across four timepoints. d) Left: number of transcript-intensity equivalents per burst at each active transcription site. Right: total number of nuclear transcripts per nucleus. * P < 0.05, ** P <0.01. *** P <0.001. e) Top: H3K27ac ChIP-seq tracks of the PPARG locus during adipogenic differentiation (range: 0-30 for all tracks); bottom: Genomic target regions labeled by DNA FISH in four colors. f) Representative maximum intensity projection of DNA FISH data (downstream control locus not shown). g) Pairwise 3D distances between genomic regions of interest measured by DNA FISH and corrected using BeadBuddy. Empirical cumulative distribution functions visualizing changes in distance between specified genomic elements as a function of differentiation timepoint (average of two technical replicates for each timepoint). h) Spatial density heatmaps: each datapoint is an absolute distance of the promoter from the midpoint of the two enhancers on that promoter’s allele. Green and purple ovals represent a distribution of enhancer positions relative to the midpoint (the origin) where each oval’s long axis is 1 standard deviation of enhancer distance from the midpoint. Detailed Statistics are presented in Supplementary Table 1.

Article Snippet: Human mesenchymal stromal cells (hMSCs, ATCC: PCS-500-011) were cultured in Dulbecco’s modified Eagle’s medium with Glutamax (DMEM, Thermo: 10-569-044) supplemented with 10% FBS (Avantor Seradigm, VWR 1500-500) and 1X penicillin-streptomycin (pen/strep) (Thermo Scientific 15140122) during expansion and for the undifferentiated condition.

Techniques: In Vitro, Cell Culture, ChIP-sequencing, Labeling, Control, Standard Deviation